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KMID : 1094720150200060988
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Biotechnology and Bioprocess Engineering
2015 Volume.20 No. 6 p.988 ~ p.994
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Non-enzymatic PLP-dependent oxidative deamination of amino acids induces higher alcohol synthesis
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Choi Kwon-Young
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Abstract
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Pyridoxal phosphate (PLP) is an organic cofactor found in all transaminase enzymes. In this study PLP was used to replace the enzymatic deamination step in the Ehrlich pathway, for the oxidative conversion of amino acids into 2-keto acids. PLP functions in an enzymeindependent manner. It was further used in the synthesis of higher alcohols through a sequential enzymatic reduction in vitro and in vivo. PLP-dependent oxidation was investigated against five representative amino acids: valine, leucine, isoleucine, norvaline, and phenylalanine. In vitro amino acid oxidation resulted in approximately 45 ~ 75% [mole/mole] of each 2-keto acid conversion and in vitro ammonia formation was less than 2-keto acid formation, with 20% of conversion yields. Whole cell E. coli expressing reduction enzymes KivD/ADH with both single amino acid and amino acid mixture (4% yeast extract) gave the highest yield (30 ~ 55%) in the presence of the PLP-Cu complex and following enzymatic reactions.
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KEYWORD
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pyridoxal phosphate, oxidative deamination, amino acids, 2-keto acids, higher alcohols
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